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lex_rooker

Lex's Protein to Fat Ratio Experiment

Lex's Protein to Fat Ratio Experiment

lex_rooker

Proposal for an Experiment

I watched the webcast of the lecture that Gary Taubs gave at the California State University at Berkley, having gotten the link from this forum.  The primary thrust of the lecture is establishing a link between eating carbohydrates and the accumulation of body fat.   I think most of us have plenty of evidence to support this general hypothesis without need for further testing.  However, one area that I’ve always been curious about is what determines our base weight, the weight that we will not drop below no matter how few carbs we eat.  In his lecture, Gary makes a provocative statement that may hold a clue.  He stated that "without glucose to cause a rise in insulin and thereby create glycerol phosphate, no body fat can be stored".  He also hinted that eating excess protein, even in the absence of carbohydrates, could raise glucose, hence insulin levels, and this might also result in some increase in body fat, though not nearly as much as directly eating carbs would.

In the absence of carbohydrates in our diet, could it be the protein level that we consume that ultimately determines our minimum or base weight?  If I understood Gary’s theory correctly, if we consume only enough protein to maintain nitrogen balance and provide the base for creating the minimum amount of glucose needed to maintain the very few glucose dependent tissues within our bodies, with the rest of our calories coming from fat alone, then our bodies will create the absolute minimum amount of insulin possible and our weight should drop to it’s absolute minimum as well.  A protein intake below this level might cause additional weight loss but would also cause us to sacrifice lean tissue to keep our bodies functioning normally.

This could explain things that many of us experience.  Our weight drops to some level, then we can eat zillions of calories and we don’t gain weight, but we find we don’t loose any either.  Might this be explained by the fact that we continued to consume excess protein, some of which is converted to glucose in the liver, which causes an insulin response that drives some fat storage – small though it may be.

My personal experience was that I started at 215 lbs, cut out all carbs, dropped to 147 lbs within about 9 months, then climbed up to 150 lbs where I was stable for several months, then my weight slowly started to climb to its present level of 160 lbs where it has remained stable for well over one year.   During this time I ate the same food with one exception.  About 18 months ago I stopped adding additional fat to my food in the form of suet. The rest of the formula remained exactly the same.  Could it be that this changed the ratio of fat to protein such that I’m now receiving far more protein than my body actually needs and therefore some of the excess is converted to glucose causing a rise in insulin and storing the excess as body fat – effectively raising my base weight by 10 lbs even though no carbs are consumed in my diet.

My current fat intake is 18% or 68% of calories, and my protein intake is about 140 grams per day and 30% of calories when I consume my normal 800 grams of food.  Total calories works out to about 2000 per day.  My weight is a very stable 160 lbs or 73 kg. I've read where the minimum need for protein is about 1.2 grams per kilogram of body weight.  At my weight of 73 kg, this would work out to a minimum of 88 grams protein per day needed to maintain nitrogen balance and properly service glucose dependent tissues.  Since I'm eating 140 grams of protein, I'm consuming well over the minimum.

If Taubs theory is correct, that body fat can't be stored without the presence of insulin, and that excess protein can cause a rise in blood glucose and hence insulin (though a much smaller rise than directly eating carbs),  I started wondering what would happen to my body fat, weight, BMI, etc. if I reduce the protein to something a bit over the minimum - say 100 grams per day, while increasing fat from 18% to 30% or about 80% of calories by adding back the missing suet to my food mixture.  Might my weight drop back to the previous stable level of 150 lbs – or even the 147 low I hit initially?

I have been eating a mono diet of the exact same food mixture every day for almost 2 years. During this time I’ve kept track of my morning ketone level, fasting blood sugar level, weight, blood pressure, etc and all have been very stable for well over a year – essentially since my weight reached and stabilized at 160 lbs.  This makes me an ideal candidate for testing Taub’s theory.

The experiment

I propose to keep the actual amount of food that I eat the same at 800 grams per day.  This is the same amount I’ve consumed since I went on the zero carb protocol over 3 years ago.  I propose to increase the fat in my diet from the current level of 18% (68% of calories) to 30% (80 % of calories).  This would increase calories from the current 2000 to 2700 which is about a 35% increase in gross energy intake.  Increasing the fat but keeping the amount of food the same leaves less room for the lean meat so protein is reduced.  My calculation based on the USDA Ground Beef calculator is a drop in protein from the current level of 140 grams (30% of calories), to about 100 grams (16% of calories) per 800 gram daily food intake.  I further propose to eat this fat enriched mixture for a minimum of 4 months (maybe forever if it works out well).  

I have a commercial UNIVEX FA-73 fat analyzer used by meat markets to determine the fat content of their ground beef.  I test the fat content of every batch of food I make and can easily stay within one half of one percent tolerance of any level between 10% and 30%.

If the (calories in) minus (calories burned) theory is correct I should gain substantial weight because over the 120 day period I will consume 60,000 extra calories without increasing my activity level.  At 3000 calories per pound of body fat, I should gain 20 pounds or about 10kg.

However, along with the increase in calories, this proposed dietary change will be reducing excess protein, which in my case is the only source for creating glucose since I consume no carbohydrates at all, and therefore insulin production should also be lower.  If Taubs is correct, with lower insulin levels less body fat would be stored, so in theory I could actually loose weight while eating all those extra calories.

I plan to document everything and post all the measurements I take on a daily basis for the forum participants to monitor and comment.  Whatever parameters I decide to measure, I will monitor and determine my current baseline before I make the actual change to my food mix. This will make it easy to monitor any deviation after the macro ratio change is made.

I don’t have any fancy lab equipment and can’t afford professional lab tests, but since we are only monitoring to see what changes, and by about how much, the blood glucose meters and urine test strips, etc available to the general public should suffice for our purpose.  Below is my current list of things I propose to track and my proposed method of measurement.  I’d appreciate any ideas for additional parameters that might be useful (along with a proposed method of measurement), or any suggestions for better measurement methods than the ones I propose.

I’d also like to get an idea of how often and when a given test should be done.  For instance, Ketones are accumulated in the urine over time and therefore measuring more than once per day may not tell you much, but the time of day that the measurement is taken may be important. I currently check ketones in first urine of the morning using Bayer Ketostix.

Blood Glucose on the other hand, may change significantly for several hours directly after eating a meal.  Therefore, testing first thing in the morning for “fasting levels” and then every ½ hour for 2, or even 3 hours after eating my daily meal might be appropriate.  Whatever is decided, this testing protocol should be run for at least a full week to establish a baseline before the change is made.  If Taub’s theory about excess protein being converted to blood glucose is right, we might see a significant difference in the after meals blood sugar curves between the high protein and high fat diets.

The other parameters on my list are:

    Fat content of every meal in grams and by % of calories (UNIVEX FA-73)
    Protein content of every meal in grams and % of calories (from USDA ground beef calculator)
    Daily caloric intake (from USDA calculator)
    Weekly 3 Skin-fold measurements Chest, Abdominal, Front Thigh (Accu-Measure calipers)
    Body weight (Soehnle digital scale)
    Urine ketone levels (Bayer Ketostix or Bayer SG10 Multistix)
    Morning fasting blood glucose levels (One Touch blood glucose meter).
    Multiple blood glucose levels after daily meal (One Touch blood glucose meter)
    Daily blood pressure (Tester at local pharmacy)


I’ve found that Bayer makes a test strip that will test for 10 different items in urine that include: Glucose, Bilirubin, Ketone, Specific Gravity, Blood, pH, Protein, Urobilinogen, Nitrite, and Leukoctyes.  If I can get these at a reasonable price I’ll add these tests to the list.

Comments and suggestions welcome,

Lex
Badger

Anthony Colpo will never accept these results.  Wink

I look forward to them greatly.  I'm excited to see what happens.  Sorry I don't have any parameter suggestions but if I come up with any I'll be sure and give my input.

Taubes has an e in his name.
Dean

Badger wrote:
Anthony Colpo will never accept these results.  Wink

Yah, he never did follow the rules here at the Metabolic Ward. He was finally locked in that padded cell, but, due to an OD of KA managed to break free. We just recently got the wall back up. Roll Eyes

After that, nobody is quite sure where he went, exactly. Initially, there were scattered reports comin' in from various neighborhoods about a naked roller skater screaming about MAD folks, but, I believe after the effects wore off he may have vanished to some unknown destination. Although, awhile back, there was a rumor floatin' around that he'd become a regular fixture on a certain city street corner, waving his "Fat Loss Bible" in the air, and proclaiming Eades is the devil. Bonkers

Poor guy. He should've stuck it out here. He has no idea how good we have it. Devil
Tracy

Lex, I think you are the (very well equipped) test subject the bus has been waiting for. This will be fascinating to follow - thanks!
lex_rooker

Tracy/Badger
I actually think this will be rather boring as most long term metabolic experiments are.   Some measurements might show a bit of action in the first week, but after that any changes should be incremental and rather slow.

As you can see from my initial post, weight changes between periods of stability took months.  Nine/Ten months from initial 215 to first stable weight of 150, and then three/four months from 150 to my current 160.

The real problem for most people is sticking with it for the long term.  I eat this way anyway so it's not a problem for me.

Lex
jem51

lex , you are the perfect candidate. i don't know when the best time is to check ketones. first thing in the morning, if you haven't vioded during the night would make them higher, i would think, since urine would be more concentrated. i think if you just stick to the same time daily, it doesn't matter....except if you eat at different times each day. maybe morning would be the best time just because of inconsistencies in your schedule.  in the hospital, glucose is tested first thing in the morning then just before meals and 2 hr post prandial. you need to check just before eating so you'll know the actual rise. of course for your own information you can check it as often as you like. i do know that some of the peop who debunk carb/no carb myths, check every 15 min so they can see the spike as well as the end result at two hours. the sticks you mention are what we use in hosps and offices so i think you should be able to get some. online if nowhere locally. do you know about how much water you consume? or is it random...just when thirsty. i think for the expirement, you should probably consume the same amt. then you can see how it effects speicific gravity, pH and all the rest. i hope others will chime in.
lex_rooker

Jem,
I always eat about the same time every day, around 4 pm in the afternoon.  

I void urine at least once during the night, and have as long as I can remember.  I have been measuring Ketones between 6am and 7am every morning depending on when I get up.

I just did my first post prandial glucose measurement.  I took 1 reading right before eating, then one reading every ½ for the next 3 hours.  Interesting results.  

Initial 1/2hr 1hr 1.5hr 2hr 2.5hr 3hr
99 102 103 106 108 109 110

It appears that when carbs are not eaten there is no traditional “spike” – just a gradual increase.  Looking at this initial data, 3 hours is not long enough to see the down side of the curve.  I’ll keep measuring every hour for a while and see what happens.  At this point I’m trying to determine what the measurement protocol should be.  Everything published is for diabetes patients so we’re forging new ground.

Found the Bayer SG10 Multstix at my local CVS Pharmacy.  Should have them tomorrow.  Just don’t know if I’ll have to get a loan on the house to afford them.

Lex
Kristelle

Upon reading your proposal, I looked back on my experiences with zero-carbing, increasing or decreasing protein/fat content. I also reread my journal. Thank god for my journal. Good thing I started one.  Wink

There was a point in time when I was feeling my best, tons of energy, ketones in urine were reducing,  body was changing for the better except that dark circles and ear blockage were still present. This point in time was when I was eating very high fat meats that contained much less protein (i.e. beef shortribs). Some of you may remember.

I know that, during that time, I never got tired after eating and eyes stopped itching and tearing for good. I slept well too.  I switched to veal a few days later as sinus problems still remained and thought it might be due to amines in beef. But, I may have switched too soon, too prematurely, as I often do (mea culpa).

But what if I was on the right track? What if too high protein and not enough fat was preventing me from keto-adapting, increasing insulin too much for me, as I was becoming very insulin-sensitve, especially in the morning, when I would often get tired after eating a high protein meal.

I now STRONGLY suspect that for most of my zero-carb diet journey, I have eaten far too much protein relative to fat, except for that short period when I felt on top of the world (read my journal). The combination of excess protein, too much insulin (and glucose) due to increased insulin sensitivity (especially in the AM) and too high ketones in my blood would explain all the symptoms I got and still get like:
hives, lethargy, drowsiness, fogginess, etc. I'm not sure about amines anymore and if anything, I expect that less insulin (and glucose) combined with a keto-adapted state would improve food intolerances/allergies as this is the case for many who low-carb.

I should have just been more patient. I was already seeing improvements but not enough for my liking. Again...impatience! The only thing was minor headaches that would last no more than 20 minutes and this, after eating almost a kilo of shortribs, usually more than my apetite would dictate. So, from now on, eat until satiated, No more!

Lex, you may have unlocked something for me and if this revelation happens to have all the positive repercussions that I expect it will have, I owe you big time!!! And I mean it. Thank you from the bottom of my Heart

And I hope that your experiment works out like you want it to and helps others in their quest to attain a healthier and happier life. Amen!

Happy  

p.s.: I'm switching back to beef shortribs.  Grin Bipolar? ME?! No!!!! You must be mistaken.  Bonkers
Badger

Long term and slow, yes.  Boring?  To some, perhaps, but I very much doubt to me.
Billi-Jean

Yipee! This is precisely the kind of experiment I would love to see! Lex, you're a gem!!  Yup

I lost weight via semi-starvation many years ago and kept it off via near semi-starvation.  Roll Eyes
So, when I switched to VLC, I did not need to lose any weight.
Thing is, because I once weighed over 80kg (176 lbs), my concern has always been that my natural 'base weight' or 'fatostat' may be at a weight that is set higher than my current weight and therefore that I would gain even on VLC.

Gaining a kilo or 2 is no concern, but, 5 or more would be a concern (I'm barely 5'1" so believe me it makes a big difference!)

I haven't been consistent for long enough at any given fat:protein ratio to figure out whether fluctuations up or down in weight are a sign of anything really, and I find it frustrating  to not be able to draw any conclusions regarding the whole excess protein issue.

Hence, this experiment could provide some pretty revealing data/results that could give people some real insight into what they might need to do if they find themselves unable to maintain their weight at a base level that they are comfortable with even on zero carb.

I will certainly be keenly tuned in to this one!  Cool
Badger

I was curious about the Univex 73 so I googled it.  Didn't find the answer to my question (how much meat does it require as a sample to analyze?) but I did find one listed on Ebay for $250.

http://cgi.ebay.com/Univex-New-Gr...015QQcategoryZ95085QQcmdZViewItem
jeff

According to the Eades, excess protein is not converted to glucose.  The body only converts what it actually needs.  Not the the Eades can't be wrong.  Does anybody have any independent info about this?  BTW, welocome Lex.
lex_rooker

Badger,
The FA-73 comes with a calibrated measuring thimble which is the black colored plastic thing directly in front of the UNIVEX label in the picture in the e-bay posting.  I'd say it is about 1 tablespoon.

The amount is very small so you must grind your sample very fine or you'll get an inaccurate reading.  A chunk of fat or lean will throw the reading off by a bunch.

The sample is heated and the drippings go into a small glass vial that has a precise internal diameter.  The scale is then adjusted up and down against the vial to measure the height of the fat in the vial.

I paid about $500 for mine new several years ago, and it came with a half a dozen measuring vials as these are delicate and will break on occasion.  The vials are the small circular things you see lined up to the left of the unit itself.

Lex
lex_rooker

My first attempt at creating an after-meal blood sugar curve has had totally unexpected results.  I expected to see a typical curve where the values started low, peaked after a couple of hours, then returned close to starting levels a few hours later.  Here I am after 16 hours and blood sugar is at 106 which is only 4 points lower than the high of 110 that was reached about 2-3 hours after the meal.  It reached 106 about 4 hours after the meal and has held steady for the past 12 hours - amazing.  I'm going to continue measuring at 2 hr intervals to see how long it takes to start dropping back to the level immediately before my meal which was 99.

The curve is very shallow which I expected, however the long stable period of slightly elevated levels is a surprise.  This may require rethinking the testing interval for Post Prandial blood glucose.  I can't afford to measure too often as the test strips are expensive, however, it appears that it's going to take many hours to record the curve and this may only be extended further when the fat is increased.

Lex
Dean

Wow, Lex, this is going to be so cool to watch. You are gonna reveal so many things.

Thanks for doin' this. Cool
Badger

Thanks for the info re: the machine.

As for the blood glucose measurements, I'd probably take a bunch of readings for a few days to see if that response pattern remains consistent, then use that data to figure out when you should take less frequent readings to get the same basic info while saving on the strips.  Probably what you were already planning on doing, but I thought 'd throw my 2 cents in.

On an unrelated note, I take it that you don't hold with Vince Garonda or any of the others who claiming that the grinding of meat destroys enzymes and other nutrients.  I've always found that an unlikely claim, but possible.

As for GNG, the Eades say that it doesn't happen, I've seen Red say that it always does, she even posted a formula in this thread:

http://activenocarber.myfreeforum...tra44308.php&highlight=#44308

I really have no idea what the truth of the matter is.
Erasmus

I think the problem variable hydration level can be mitigated by the morning reading.  That reading will be high due to the body being at its lowest hydration level unless water is taken in during the mid-night void.  So, while the reading will be high, it will still be valid for trending purposes.

Useful would be (possibly) using a bodyfat measuring scale that does hydration levels.  

How long do you plan to do baseline measurements before you start the experiment?  I would think a full week to account for weekly variables a minimum.  

I gotta say kudos and way cool, by the way.  Thanks for starting this up.  I really look forward to your data.

And lastly, you've shamed into doing something I've wanted to do for a long time.  $500 for a fat tester?  I guess I need to go ahead and get that magnetic stirring hot plate that I've wanted for so long.  Bonkers

-E
lex_rooker

Well there may be trouble in paradise.  I’m having trouble getting consistent readings of blood glucose levels.  I’ve tried 2 different meters: FreeStyle Freedom & OneTouch Ultra2. I tried 4 successive measurements with each meter.  The results varied by as much as 10 points for both meters.  The FreeStyle gave readings between 99 and 108.  The OneTouch gave readings between 92 and 103.

These are fine for diabetics, but may be of limited use for monitoring small variations in blood glucose levels.  I even tried a second run with different vial of test strips with similar results.

Any ideas for getting consistent readings?  Anyone know of a meter that is known for it’s accuracy?

Help would be appreciated,

Lex
Erasmus

Wow.  A quick google comes up with that the FDA requires a 20% or better margin of error.  Wow

Sad.  That you are at 5% is pretty good.  

I suggest that you just note the margin of error and continue.  Sadly, if your experiment shows a reliable number under that 5%, we'll never know.

-E
lex_rooker

I ran across the 20% number also.  If the error was related to a calibration standard, but consistent with every measurement, it wouldn't be a problem.   The issue I'm seeing is up to a 10% difference between consecutive readings on the same meter taken approximatelly 15 sec apart - as fast as the meter will cycle.

It could be my technique.  In the past I've only taken a few readings per week and never consecutive readings so I'd never see the problem.  I'm re-reading the manual and making sure I follow the insturctions to the letter. We'll see if this (and a bit more practice) helps.

Lex
jem51

lex, i think you just need to pick a tester and stick w that particular one.  i have read that meat/fat eating has that effect on blood sugar; small rise that stays steady for a long while. are you doing a morning reading just to see what it is after the night? does the lower number remain about the same as pre-meal?  i'm not sure that the body adapts to using ketones to the point of none in the urine. if we use all we need and there are some left over, then obviously they'll be excreted. i would think that if you have eaten this way for several years, and complete keto adaptation theory is true, you would have no ketones....i've been talking about picking up a glucosemeter for a couple years now...guess i'll go do it.
lex_rooker

More on the meter issue.  Using the FreeStyle, I tried the forearm to see what kind of reading I'd get there.  Took 2 readings an hour a go a few seconds apart and one was 106 the other was 107 - thought I hit paydirt!

Well time for another hourly reading.  Same meter.  Same method - fresh blood draw for each test from the same site.  This time first reading was 111 and the second reading 15 seconds later was 103 - not good.  This is the same problem I'm seeing when using a finger for the blood source.
lex_rooker

In trying to get as much background as I can for this experiment, I spent some time reviewing a thread in the Carnivore Cafe where Dean summarized some of the Bear's wisdom on creation (or not!) of glucose from protein.  If I understood the Bear correctly, he was adamant that under normal conditions (not in starvation mode), little or no glucose is needed by someone well adapted to a no-carb diet, and any glucose that may be necessary will be created from glycerol that is available in the blood stream and liver as a by-product of normal fat metabolism.

I am seeing a small rise in blood glucose, say 10%, after eating my daily meal.  This then tapers off to about 5% above the low about 5-6 hours after eating and stays there for 12-15 hours before dropping to a low an hour or two before my next meal.  The entire cycle is taking 24 hours.

I seem to be having some consistency problems with the glucose meters but this is a trend I'm seeing after 2 days of measuring.  If, as the Bear says, there is no "automatic" conversion to glucose of either fat or protein, then what is causing the glucose to rise after a meal when no carbs are eaten?  From Bear’s comments, I wouldn’t expect any rise in blood glucose at all, “as any glucose deficiency would be immediately made up as it occurred, by conversion of glycerol which is readily available at all times in the blood stream and liver” (per the Bear).

Even if you take into account that there is a small level of carbohydrate in some of the organ meats that I eat, I would expect them to metabolize quickly like any other carb source, and I wouldn't think there would be a sufficient quantity (5-10 grams at most) to keep blood glucose levels elevated for 18 to 20 hours.

Also, if the Bear is correct, then I don’t believe I should see any change in any parameter that I’d be able to measure when I change the fat/protein ratio of my daily meal.  Bear believes all blood glucose would be coming from glycerol as a by-product of fat metabolism, and all excess protein eaten is just flushed out by the body.  As long as I’m getting “enough fat” and “enough protein” in my current food mix to satisfy my “fatostat”, then any excess of either nutrient will just be discarded.  Changing the ratio should have no effect whatsoever.

Wish I could get good consistent readings from a glucose meter.  The ability to check this value accurately might be very important to this experiment.

Thoughts?
Badger

http://www.healthboards.com/boards/showthread.php?t=396174

This thread has a lab technician who is frustrated with the inaccuracy of these tests looking for the most accurate one.  Various people recommend different meters as having been accurate for them relative to the lab test.  Lots of technical discussion.  You may find it interesting.
Dean

I thought Bear was only talking about protein not being used for GNG. While he thought glycogen stores had a very slow turnover, I don't recall him saying that all dietary fat was flushed from the system. One possible explanation of the small rise in BG is that dietary fat is being converted by the liver via GNG and the glucose produced is being released to be absorbed by muscle glycogen stores.

The dispersal of glucose in both liver and muscle glycogen stores is critical to keeping blood glucose levels at a certain range throughout the entire body. Obviously the extra glucose is coming from somewhere, and that somewhere is more than likely your food intake. It will be interesting to see how the various macro ratios affect the amount of the rise in BG levels. One thing you could try is to eat lots of protein with little fat, and then see what your BG levels are. If Red is correct, your BG levels would go way up. My money is on them staying the same as they have been. Only carb intake is gonna drive BG up to really high levels. Not a very desirable situation, if you ask me.
lex_rooker

Badger

Thanks for the link.  I'll investigate today.  First blood Glucose readings this morning were all over the map.  108, 104, 114, 100.  This isn't good enough with the small BG swings I'm experinecing.

Dean,

I'll go back and look at the thread again, but I came away with the understanding that both unused fat and protein were discarded.  If this is not true, and we overeat fat calories necessary to maintain body mass, then why don't we gain lots of weight?  That energy has to go somewhere.

Lex
Erasmus

lex_rooker wrote:
I am seeing a small rise in blood glucose, say 10%, after eating my daily meal.  This then tapers off to about 5% above the low about 5-6 hours after eating and stays there for 12-15 hours before dropping to a low an hour or two before my next meal.  The entire cycle is taking 24 hours.

I seem to be having some consistency problems with the glucose meters but this is a trend I'm seeing after 2 days of measuring.  If, as the Bear says, there is no "automatic" conversion to glucose of either fat or protein, then what is causing the glucose to rise after a meal when no carbs are eaten?  From Bear’s comments, I wouldn’t expect any rise in blood glucose at all, “as any glucose deficiency would be immediately made up as it occurred, by conversion of glycerol which is readily available at all times in the blood stream and liver” (per the Bear).

Even if you take into account that there is a small level of carbohydrate in some of the organ meats that I eat, I would expect them to metabolize quickly like any other carb source, and I wouldn't think there would be a sufficient quantity (5-10 grams at most) to keep blood glucose levels elevated for 18 to 20 hours.

Thoughts?


This is strictly off the top of my head, so maybe it's insane...  Devil

The act of digestion spawns a wide variety of physiological responses.  MAYBE an up-tick in blood glucose is one of those responses.

-E
lex_rooker

I read the post regarding Accuracy/Reliability of Glucose Meters for home use that Badger provided.  Looks like  the absolute best we can expect is about a 5 point difference between consecutie measurements.  Following the recommenadations of how to take the measurement did allow me to make several measurements that where the max difference was 8 points between lowest and highest, but most  readings were about 4 points apart from one to the next: I.E. 95, 101, 97, 93

My BG only rises about 10 points after a meal.  With an average 4-5 point spread possible between readings, looking for a change of 10 points, the error in the reading could easily be off by 25%-50% of the total move. This is not overly useful, but it appears to be the best we've got.  

Oh well, we may be able to see general trends.

Lex
Erasmus

You could do multiple reads and employ some nasty, long hair, black magic statistics to reduce the error.  

Or just multi read, use the average and call it a day. After all, it's not going into pubmed.   Wink

-E
Dean

lex_rooker wrote:
If this is not true, and we overeat fat calories necessary to maintain body mass, then why don't we gain lots of weight?  That energy has to go somewhere.

Futile cycles.

http://magicbus.myfreeforum.org/sutra10416.php#10416
Dean

Based on the glucose concentration in the blood, the pancreas will constantly release insulin and glucagon. These two hormones will in turn regulate hepatic GNG. So, insulin will tell the liver to stop producing glucose and glucagon will tell the liver to start producing glucose. Turning off and on GNG is how proper blood glucose levels are maintained. The range is very critical.

The glycogen stores in the muscles and liver are reserves for blood glucose, so they will need to get replenished. When you remove the concept of dietary glucose, it is easier to make sense of what is going on. Our blood cells must have glucose. Our liver makes this glucose via gluconeogenesis (GNG). This GNG is regulated (turned off and on) by insulin and glucagon, which our pancreas creates and releases based on the concentration of glucose in our blood. The glycogen stores will take in so much of this glucose (to keep their reserves at a certain level) and then stop taking it in. The system works great.

Once you introduce dietary carbs, your glucose levels rise out of sight, and your pancreas starts losin' it. "Oh, my god, we have to start puttin' out insulin to go tell the liver to STOP making glucose! STOP that GNG!" This is what is happening. Cells don't need insulin to uptake glucose, and excess glucose will get sucked into cells and burned up, just to get the excess glucose out of the blood. This is not a good thing for the cells, however. Free radicals are the end result of this type of emergency metabolism. One of the byproducts of this glucose metabolism in the cells is glycerol phosphate. This glycerol phosphate will aid fat storage. That's how eating carbs will make you fat.
lex_rooker

I've read the post on futile cycles and still don't quite understand how this could happen without signifcantly raising body temperature or creating a sweat reaction to keep core body temperature stable. Just minor exercise, burning an extra couple of hundred calories per hour, will cause noticable sweating as the body attempts to keep it's internal temperature stable.

As I understand it, metabolic functions & reactions are exothermic and so give off heat.  It just seems that doing enough (futile?) work to consume several thousand excess calories per day without generating additional heat equivalent to consuming the same number of calories through exercise is violating some law of physics.  Work is work.... or is it?

Lex
Erasmus

lex_rooker wrote:
I've read the post on futile cycles and still don't quite understand how this could happen without signifcantly raising body temperature or creating a sweat reaction to keep core body temperature stable. Just minor exercise, burning an extra couple of hundred calories per hour, will cause noticable sweating as the body attempts to keep it's internal temperature stable.

As I understand it, metabolic functions & reactions are exothermic and so give off heat.  It just seems that doing enough (futile?) work to consume several thousand excess calories per day without generating additional heat equivalent to consuming the same number of calories through exercise is violating some law of physics.  Work is work.... or is it?


Work or entropy, you get your choice.   Devil

You are right FCs produce waste heat.  
Assuming 1000 XS heat Calories (kcal):

Heat of Vaporization of water:
965 btu/lb or 243 kcal /lb

So it would seem we would need about 4 pound of sweat to dissipate that much heat.  Pints a pound the world around (grandma Happy ).  Thats half a gallon.      

I know I don't sweat that much.  Unless I'm watching other people work that is.  LOL

I suspect we excrete much of it.

-E
Dean

You know, that's what a lot of us were saying back when jeff did that high fat week. We suspected excretion. The gall bladder can only deal with so much fat, so, there is probably only so much that gets digested at one time. However, if there ends up being any excess fat digested, then futile cycles could use that without it going to fat stores. FFAs get released into the blood from the lymph system. I'm sure there is a limit to how much fat can get absorbed into the body and put into that lymph system at one time. Lex, why don't you eat 80,000 calories of fat throughout the day and see if you catch on fire? Shock  LOL
lex_rooker

Dean wrote:
You know, that's what a lot of us were saying back when jeff did that high fat week. We suspected excretion.

Hmmmm, I think I said something like that a couple of posts ago....
lex_rooker wrote:
I'll go back and look at the thread again, but I came away with the understanding that both unused fat and protein were discarded.

Discarded/Excretion - Time to pull the old FUNK & WANGELS out of moth balls.
Dean wrote:
Lex, why don't you eat 80,000 calories of fat throughout the day and see if you catch on fire?

When I move to 80% fat, I'll be sure to sleep in one of those Oily Rag containers we had in Auto Shop - you know, the ones designed to stop spontaneous combustion.
lex_rooker

I'm making some progress with getting more consistent readings on the glucose meters.  There is a bit of technique required.  Now most of my consecutive readings are within a couple of points and that's a good thing. Once in a while there's an outlier, but I think the situation is managable.

When changing to a new batch of test strips there's a big jump in readings but they seem to stay realtively consistent within each batch.  Trouble is I'm burning through them at the rate of about 30 per day so a bottle of 50 lasts about a day and a half.  Hope to get about 3 or 4 days out of 50 once I get the testing protocol down.

Lex
Dean

lex_rooker wrote:
Dean wrote:
You know, that's what a lot of us were saying back when jeff did that high fat week. We suspected excretion.

Hmmmm, I think I said something like that a couple of posts ago....
lex_rooker wrote:
I'll go back and look at the thread again, but I came away with the understanding that both unused fat and protein were discarded.

Discarded/Excretion - Time to pull the old FUNK & WANGELS out of moth balls.

OK. And, I was responding to E, who said:
Erasmus wrote:
I suspect we excrete much of it.

So, looks like we both might agree with you. Wink

Actually, I don't really have much of an opinion on it. It's just that we were throwing that out as one possible explanation back during one of jeff's experiments.

lex_rooker wrote:
It just seems that doing enough (futile?) work to consume several thousand excess calories per day without generating additional heat equivalent to consuming the same number of calories through exercise is violating some law of physics.

Here's what Eades says. BTW, he doesn't agree about the fat being excreted.

Eades wrote:
First, I don’t think they go out in the bowel. If they did, people would have cosmic pizza grease stools whenever they ate a lot of fat over a period of time, and they don’t. And a number of studies have shown that increasing fat in the diet doesn’t increase fat in the stool.

Eating a very-low-carbohydrate diet ensures that insulin levels stay low. Unless insulin levels are up, it’s almost impossible to store fat in the fat cells. With high insulin levels fat travels into the fat cell; with low insulin levels fat travels out. So, it’s pretty safe to say that the fat isn’t stored. So what happens to it?

The body requires about 200 grams of glucose per day to function properly. About 70 grams of this glucose can be replaced by ketone bodies, leaving around 130 grams that the body has to come up with, which it does by converting protein to glucose and by using some of the glycerol backbone of the triglyceride molecule (the form in which fat is stored) for glucose. If one eats carbs, the carbs are absorbed as glucose and it doesn’t take much energy for the body to come up with its 200 gram requirement; if, however, one isn’t eating any carbohydrates, the body has to spend energy to convert the protein and trigylceride to glucose. That’s one reason that the caloric requirements go up on a low-carb diet.

The other reason is that the body increases futile cycling. What are futile cycles? Futile cycles are what give us our body temperature of 98.6 degrees. Futile cycles are just what the name implies: a cycle that requires energy yet accomplishes nothing. It operates much like you would if you took rocks from one pile and piled them in another, then took them from that pile and piled them back where they were to start with. A lot of work would have been expended with no net end result.

The body has many systems that can cycle this way, and all of them require energy. Look up the malate-aspartate shuttle; that’s one that often cycles futilely.

Another way the body dumps calories is through the inner mitochondrial membrane.

So, it appears this idea of futile cycles is not some abnormal thing. Looks like the body does this as part of it's regular metabolism. I suspect if it didn't, our body temps would drop so low we'd die. Our normal temps are up because of this futile cycling, as well as all the other exothermic reactions taking place.

Eades wrote:
You can see that the whole process requires some means of regulation. If not, then the electromotive force (called the protonmotive force, since it’s an unequal concentration of protons causing the force) can build up to too great a level. If one overconsumes food and doesn’t need the ATP, then the protonmotive force would build up and not be discharged through the turbines because the body doesn’t need the ATP. The body has accounted for this problem with pores through the inner mitochondrial membrane where protons can drift through as the concentration builds too high and by proteins called uncoupling proteins that actually pump the protons back across. So we expend food energy to pump protons one way, then more energy to pump them back.

One of the things that happens on a high fat diet is that the body makes more uncoupling proteins. So, with carbs low and fat high, the body compensates, not by ditching fat in the stool, but by increasing futile cycling and by increasing the numbers of uncoupling proteins
and even increasing the porosity of the inner mitochondrial membrane so that the protons that required energy to be moved across the membrane are then moved back. So, ultimately, just like the rocks in my example above, the protons are taken from one pile and moved to another then moved back to the original pile, requiring a lot of energy expenditure with nothing really accomplished.

I don't know, guys. But, one thing I do know is I've been consuming TONS of fat calories for quite sometime, and I haven't gained one gram of body fat. So, where did all this ATP go? Moving protons? Perhaps.

Only the black box knows for sure. Wink
jeff

Dean wrote:
I don't know, guys. But, one thing I do know is I've been consuming TONS of fat calories for quite sometime, and I haven't gained one gram of body fat. So, where did all this ATP go? Moving protons? Perhaps.

Only the black box knows for sure. Wink

That's my theory exactly!   LOL   I find this stuff interesting, but I don't lose sleep over it.
Heather L

jeff wrote:
Dean wrote:
I don't know, guys. But, one thing I do know is I've been consuming TONS of fat calories for quite sometime, and I haven't gained one gram of body fat. So, where did all this ATP go? Moving protons? Perhaps.

Only the black box knows for sure. Wink

That's my theory exactly!   LOL   I find this stuff interesting, but I don't lose sleep over it.

Funny thing is I do lose sleep over it... literally.  With LC if I have many more calories than I usually do, I wake up in the night hot enough not to need any blankets.  This doesn't help my sleep much. LOL   I know this isn't hot flashes, as it did not occur before LC, and only happens when I have overdone the amount of food.   Does anyone else experience this?  Maybe I am the only "lucky" one to notice it.
Valcan

Lex,

Great experiment, I will be reading your updates daily.  You can get the urine strips you mentioned near the beginning of this thread at Amazon for $37.75:

http://www.amazon.com/dp/B000ND7Q...=dealtime-hpc-20&linkCode=asn

Val
lex_rooker

Valcan - thanks for the link.  Much less than half the price I paid at my local pharmacy.  I knew I could get them cheaper on-line but didn't want to wait for a week or so for shipping.  Will definately re-fill through Amazon as that is the cheapest I've seen  them.

Futile wars - Worrying over futile cycles is futile.  No way to measure them or even know what they directly or indirectly affect.

Lex
lex_rooker



I have some preliminary data on my Blood Glucose levels.  I took measurements every ½ hour from the start of my meal at 1600hrs (4pm) continuing for 3 hrs until 1900hrs (7pm). From 1900hrs (7pm) until 2200hrs (11pm), I took a measurement every hour. Upon arising at 0700 (7am) until my meal at 1600hrs (4pm) I measured every 2 hrs.  The readings were very stable starting 3 hours after eating until about 3 hours before eating.  I only measured a couple of times in the middle of the night, and the readings were consistent with the readings taken just before bed time and the first reading of the morning so I took the liberty of filling the night hours with the average.

The data was collected over a 4 day period and I averaged the 4 different readings of each hour of the day to smooth the data.  This gave me numbers that had a decimal point and I rounded these up or down to the nearest whole number

What is interesting is that on a relatively high fat diet like I eat, my blood glucose levels don't fall to "fasting" levels for about 20 hours after eating.  I eat my meal at 1600 hrs every day.  The BG peaks within 1 hour and the peak at around 109 is less than 5 points above the normal average of 106.  Within 3 hours it has dropped to its average level of 106 and stays there for the next 18 hours.  At about 1300 my BG rapidly drops 10 points to 95 or so until I eat again at 1600 hrs and the process starts over.  Fascinating.  What a difference from the yo-yo effect we see from eating a high carb diet.

I've taken almost 100 readings over the last 4 days so my fingers are feeling a bit abused, but I think it's worth it as I've never seen data like this before.

Lex
jeff

Very cool.  Thanks Lex.
Badger

Beautiful.  You are probably not diabetic.  LOL

Though, according to the ADA a "fasting" glucose reading can be taken as little as 8 hours after eating.  The result of 106 that you get at that time says that you have prediabetes with a risk of actually getting diabetes.  I suspect that this is not the case and that the diagnostic standards are simply not applicable to a ZC diet.

Moving forwards, what's the next step?
lex_rooker

Badger,
I assume you are correct that a high fat, zero carb diet doesn't fit the blood glucose curves caused by the Standard American Diet.  However, it is clear that with what I am eating today, there is a "fasting" level, it just takes over 20 hours to for my body to return to it.  I usually start getting hungry between 1pm and 2pm and it looks like the blood sugar curves explain why.  I'll also be showing this to my doctor who wants me on Metformin becasue the lab values for my blood tests taken at 10am with no food intake since my meal the previous day indicate pre-diabetes.  Wonder if there will be any significant change when I up the fat and reduce the protein?

What's next?????

Right now I'm measuring about 20 differenet things, many of which don't seem relevent, so the first step is to figure out what is relevent and reasonable to measure as well as the measurement protocol.  Then I need to make sure I've captured a good baseline for each paramenter measured.  The hardest part for me is to work out the best approach to dispaly the captured data so that daily changes can be evaluated against the base line and/or a longer term trend.

It really is a lot of work because I've never made many of these measurements before so getting consistent results presents a significant challenge.  I've never done much with Excel so I'm having to do a lot of experimentation to get things to work at all - much less the way I want them too.  I'm wearing out the F1-Help key on my PC.  There is also the learning curve of actually getting the stuff posted to to the Forum which is completely new to me.  Thank goodness I have  Dean to help me through the muck and mire of this primordial ooze.

Lex
Erasmus

You could just use google docs and post all the data.   Grin

-E
Valcan

Hi Lex,

If you are having problems with formulas in excel, here is a video to help:

http://www.teachertube.com/view_video.php?viewkey=627e85f85fa328bfb59f

If formula's are not what is causing you grief, just search on Teachertube until you find what you want.  Lots of great videos to learn from.

Val
Heather

So, on the 20th hour the BG drops 10 points in one hour? Is that a normal drop? I thought it would be more gradual slope. Interesting....
Dean

Here's a thought.

What if Lex's body is so used to him eating on this schedule, that it has become adapted to doing GNG over those 20 hours, and distributing the glucose to the various muscle glycogen reserves throughout his body, and it knows just how much to make, and has determined the rate at which to make it, then it stops and waits for him to eat again, therefore supplying more fat or protein for GNG? The body always tries to find the most efficient ways to do things. Hence the reason for randomization with eating and exercising to throw off the body, to force it to try to adapt. Our ancestors randomized eating times naturally, since the food supply was a bit erratic at times. Calorie cycling may just be the ticket to shedding pounds on zero carb. Huh? Maybe I'll try it.
Heather

That is an interesting thought!
Billi-Jean

These results make sense to me. If a drop in blood sugar is a primal driving factor for making one seek out food, then this 'sudden' drop (though it doesn't seem excessive to me) at the 20 or so hour mark, would ensure that one gets quickly motivated again to eat. In conjunction with the stress hormones produced concurrently, it all seems to lead, as far as I can tell, to conditions that yell, "GO HUNT damn you!"  LOL
lex_rooker

Heather,
I'm not sure exactly how long it takes for my blood sugar to drop.  I only measure it once every 2 hours and I find that at noon it is at the higher level and then in the 4 hrs before eating it drops to the 'fasting' level.  The 2pm reading may be the higher level, the fastiig level, or somewhere in between.  The chart is just an average over several days.

Ive also noticed that mild exercise has some effect.  If I walk 2-3 miles round trip to the bank or market in the early afternoon then the blood glucose drops eariler than if I'm sedentary.  One day I didn't do anything but lay around reading a book and my blood glucose stayed at the higher level all day.

Lex
lex_rooker

Thought I better clarify a statement in my last post about measruing my blood glucose levels every 2 hours.  

I've actually been measuring every 1/2 hour directly after eating for 3 hours and then every hour until I go to bed.  I measure upon arising (about 6 am) and then every 2 hours until I eat again.

That's about 18 measurements per day and I have to take 2 or more readings on occasion if I get a reading that seems out of line.  I go through about 50 test strips in 2 days.  That's a lot of holes in my fingers.

My goal is to reduce the testing to as few times per day as possible and still get a useful picture of blood glucose levels throughout a 24 hour period.  To do that I have to test more often in the beginning to see what the general pattern is.  Then I can reduce it to less often to verify that the pattern is still holding.  I'll take the same approach when I change to the higher fat meals - I'll test more often in the beginning until I establish a pattern and then reduce testing to monitor that the pattern is stable.  If there is any deviation I'll resume more frequent testing again.  

Tryig to keep from shredding my fingers as much as possible.  They really do become brused and painful with this amount of testing.  Hope this clarifies,

Lex
SandyDown

lex_rooker wrote:
Heather,


Ive also noticed that mild exercise has some effect.  If I walk 2-3 miles round trip to the bank or market in the early afternoon then the blood glucose drops eariler than if I'm sedentary.  One day I didn't do anything but lay around reading a book and my blood glucose stayed at the higher level all day.

Lex


Very interesting info Lex, thanks for sharing :)
Bobi

Why are you testing so often?   Maybe I missed a previous post, if so, excuse my questions.  Are you a complicated diabetic?  You must have no available finger tips left!!!  50 times a day seems to be kind of extreme.  All I can say is OUCH!!! Yup
lex_rooker

bobi,
I'm averaging a little over 20 times per day, some of it due to having to take multiple readings if one seems way out of line.  You get a bad test strip now and then and also, I've found that if there is anything at all on your fingers the reading become very eratic.

The idea is to get a clear picture of what happens to my blood sugar levels throughout the day and see if it changes when I change the fat to protein ratio.  Once I have a good picutre of the pattern I can reduce the testing frequency significantly.

Take a look at the chart at the top of this thread.  This blood glucose profile isn't anything like what is seen when eating a normal high carb diet.  Without the frequent testing I wouldn't have seen this.

Lex
Bobi

Aw shucks, Lex...I missed the whole first page of the Thread.  My apologies. Oops
Heather

Thanks for the info, I really find this fascinating.  Yup
Tracy

So do I - following along with great interest :)
Jude

Heather L wrote:
jeff wrote:
Dean wrote:
I don't know, guys. But, one thing I do know is I've been consuming TONS of fat calories for quite sometime, and I haven't gained one gram of body fat. So, where did all this ATP go? Moving protons? Perhaps.

Only the black box knows for sure. Wink

That's my theory exactly!   LOL   I find this stuff interesting, but I don't lose sleep over it.

Funny thing is I do lose sleep over it... literally.  With LC if I have many more calories than I usually do, I wake up in the night hot enough not to need any blankets.  This doesn't help my sleep much. LOL   I know this isn't hot flashes, as it did not occur before LC, and only happens when I have overdone the amount of food.   Does anyone else experience this?  Maybe I am the only "lucky" one to notice it.


Well, having been generally LC for 10 years now, and more relevantly, from pre-premenopausal, through an accelerated menopause and out the other side (I'm now officially post-menopausal), I think I can speak to that assumption a bit.

My experience has always been surges of hormone related activity, particularly estrogenic I think, on going through the (my term here, for lack of any other) "ketonic" process.  I've always had symptoms directly attributable to an estrogenic surge (very probably in some feedback loop relation to the other 4 major endocrine hormones) at the point in time where I have gone "back" to a VLC diet after waffling ;) for a time.  These symptoms range from breakthrough bleeding (even when under hormonal "control" such as on BCPs) to hot flashes and/or flushing.  Still get the hot flashes and flushing on occasion.

Bottom line, it may not be "menopausal" hot flashes, but hot flashes aren't reserved for menopause...

How fun, to not fit on the normal part of the bell curve of medical test reference ranges, which we do well to remember is merely the collected data of all medical tests done, and the vast majority of those are on people eating the "normal" food of the USDA mandated S.A.D.  Sigh.
lex_rooker

Time for an update.

My wife’s two sisters are going on a tour of the Eastern Seaboard on May 1st and my wife has decided that we should join them for on this two week sojourn.  When away from home I have no problem keeping to my dietary rules, however, I have no way to accurately measure what I’m eating so unfortunately I’ll have to delay the start of my experiment until I return.

We will be leaving April 30th, and returning May 15th.  I will re-establish a baseline between May 15th and May 30th to assure that it is in line with where I am today, and then make change to a higher fat content on June 1st.

This came out of the blue.  The wife and her sisters just came up with this last week and it wasn’t finalized until they obtained all the airline tickets, rental car, and hotel reservations over this weekend.  

I’m a bit disappointed but am committed to seeing this thing through.  In the mean time, for the rest of this month I’ll be working on finalizing just what I will measure and the measurement protocols.  I can then hit the ground running upon my return.
jeff

Sounds good, Lex.  I hope you have a great trip.
lex_rooker

In an earlier post on this thread I think it was Dean who mentioned that our ancestor’s would not have eaten on a schedule as their life would have been much more chaotic. As I remember there was a suggestion that my body my have adapted to my eating schedule.  I totally agree with this assessment so decided to see what would happen if I incorporated a more flexible schedule into my routine.  

Last week I tried a bit of this to see what would happen.  I ate a meal at 4pm on one day and 11am the next.  There were only 19 hours between these meals and BG never dropped to fasting levels. I ate late the following day so there was more than 24 hours between the meals.  Measurements were all over the map and it was impossible to determine what was normal much less what changed.

All I can say is that it doesn’t seem very conducive to achieving consistent results for an experiment like the one I’m running.  Eating at different times means that BG will ebb and flow at different times which makes seeing any pattern difficult.  This difficulty could be somewhat overcome by keying all readings to meals rather than a specific time of day, but you still run into the issue of not having a consistent time between meals so BG doesn’t establish a reliable pattern.  


The idea of random eating times doesn’t seem practical for what I’m attempting to do.  If anyone has a suggestion let me know.  Since my experiment has been delayed by about a month I have time to test some ideas before I start setting the baseline on May 15th.

Lex
Karen

I am excited to see your 4 mos results Lex. I am especially interested in any weight/gain or loss over that time and whether you will drop that 10 lbs even though eating 700 extra cals per day. I am impatient..so this will be a LONG wait..lol..but THANKS for doing this lex!
Billi-Jean

I'll echo the others -I'm glad you're still going ahead with this! Cool

As a side note relating to the earlier talk about glucose levels after the meal and whether enough protein can trigger insulin release...

Something interesting that I picked up when studying for my endocrinology exam -regarding the pancreas:

The 'Tortora' textbook clearly acknowledges that the 4 different pancreatic hormones -glucagon, insulin, somatostatin and pancreatic polypeptide, and their interactions, are very complex and not well understood! (This makes me think of Bear and his comments constantly that much of our 'understood' or accepted metabolic processes, as described and accepted as fact, are not in fact proven as acting in the body the way medical establishment and physiology textbooks insist they do because it is very difficult to establish in vivo methods to ascertain their functions and interactions.)

The textbook also makes a point of noting that while insulin secretion will be stimulated by 2 of the amino acids in protein, this won't happen if blood glucose is low (ie if no carbs are eaten with the protein), because the increased blood levels of amino acids that occurs after a meal with mostly protein trigger glucagon release instead of insulin.

So it seems that protein will only trigger insulin secretion if you eat it as part of a mixed meal with carbs.
lex_rooker

Karen - sorry I have to delay the start of this project, but to try to measure things accurately while having to accommodate the dining whims of 5 other people would be almost impossible. It will be all I can do just to stick with my meat-only protocol.  Eating once a day helps here.  Usually there is at least one restaruant we hit durring the day that has something appropriate on the menu, and if not, I hit the local meat market for a couple of pounds of ground chuck.

Billi-Jean - very interesting.  I've often questioned the Bear's pronouncements for the very same reason he himself finds the current medical wisdom suspect.  I'm just no sure anyone one really knows exactly what's going on inside our bodies.

The best we can do is track the few things that we can objectively measure and see if we can find a reliable (and repeatable) cause-and-effect relationship.

Personally, I consider what is happening at the cellular, hormonal, and mitochondrial levels as magic.

Lex
Billi-Jean

I agree Lex.  Yup  And Bear's conclusions are subject to the same limitations, of course. But I just think that this is interesting stuff in light of the recent concerns regarding protein consumption and the claims regarding how much of it is supposedly converted to glucose and if, when or how it triggers insulin and such...

I'm fascinated by your experiment because you don't eat carbs. This introduces parameters that I believe are more relevant to carnivores and have a better chance of revealing more accurate relationships than we usually see in scientific studies pertaining to how different macronutrients are handled in the body under conditions which don't involve carbohydrate ingestion, and thus the subsequent biochemical 'effects' they then produce.
Kristelle

If I recall correctly, Lex includes in his daily diet some organ meat (25%) which contains some carbs in the form of glycogen. So, it's not 100% zero-carb but pretty close to.
Nicola

All meat has glycogen - not just organs.

I asked Dr. Groves and he thinks that the glycogen in meat is no problem; he says that he burns ketons and he is eating eggs, cheese, cream, meat and even a little fruit.

Yes, all carnivores must use some ketone bodies for energy. While certain body
cells rely on glucose, there would not be enough glucose generated from the
glycerol in triglycerides, that is a minor source. Ketone bodies serve the same
purpose as glucose in fat-adapted humans and animals.

I am not sure about ketosis in babies. Human breast milk is relatively high in
sugar, because a baby is adapted to put on fat to maintain a stable energy
supply during periods when foods might be scarce. But this could mean that,
while breast feeding they won't be in ketosis.

I am in ketosis and have been for years.

Barry Groves, PhD


A nother answer on the rawpaleo group was:

I've thought about glucose from protein regarding rabbit starvation.
If all that protein were really used to make glucose, people could get
fat on a diet of lean meats but instead, they waste away and diet even
faster than by regular starving. I don't see a change in my BG after
meals.

People who were doing the Kimkins diet, which is a high protein, very
low carb and very low fat diet, reported improvements in their
diabetes. This diet induces rabbit starvation and is why people lose
weight so rapidly but many also become ill, their hair falls out, ect..

My guess is, and I've read this before, that protein is used for
building and maintaining lean body mass, some is used for
gluconeogenesis but only enough to maintain normal blood glucose
levels and the rest is discarded. Kimkins dieters do go into ketosis
but they are burning only body fat for energy which is why they lose
so much faster than high fat low carbers. If continued, no doubt they
would die but when they reach goal wait and go onto maintenance, they
add some carbs and fat. It's a very controversial diet.

Glycogen stores are used up in about two or three days so you would
have to eat about 1/3 your lean body weight in lean meats every day to
keep your glycogen stores up enough to prevent ketosis from setting
in. If your lean body mass is 100 lbs, you'd have to eat about 33
pounds of meat per day. Even with the higher glycogen stores in horse
meat, it'd be impossible to eat enough to prevent ketosis.

Here are a couple of studies re: blood glucose and protein:
http://tinyurl.com/3q6r6s
http://tinyurl.com/6tq9c

Craig

And I found this posted on the Magic Bus:

The 'Tortora' textbook clearly acknowledges that the 4 different
pancreatic hormones -glucagon, insulin, somatostatin and pancreatic
polypeptide, and their interactions, are very complex and not well
understood! (This makes me think of Bear and his comments constantly
that much of our 'understood' or accepted metabolic processes, as
described and accepted as fact, are not in fact proven as acting in
the body the way medical establishment and physiology textbooks
insist they do because it is very difficult to establish in vivo
methods to ascertain their functions and interactions.)

The textbook also makes a point of noting that while insulin
secretion will be stimulated by 2 of the amino acids in protein, this
won't happen if blood glucose is low (ie if no carbs are eaten with
the protein), because the increased blood levels of amino acids that
occurs after a meal with mostly protein trigger glucagon release
instead of insulin.

So it seems that protein will only trigger insulin secretion if you
eat it as part of a mixed meal with carbs.

So what is one to think?

Nicola
Billi-Jean

Thanks for the links to those studies, Nicola. I think they are a good example of the parameters I was alluding to (ie: regarding the limitations of studies purporting postprandial effects of protein on glucose and insulin levels).

The diabetes journal article claims to measure glucose/insulin etc. in low carb diets, but the carbohydrate:protein:fat ratio of the test group was 20:30:50, which is still a decent amount of carbs in my book and thus does not create parameters that would necessarily reflect conditions in a zero carb, keto-adapted individual. Sure the glucose/insulin response seemed favourable (ie: decreased), but it doesn't really tell us what happens to protein in the absence of carbs.

Similarly in the second study which was reporting effects of increased protein rather than low carb (30:40:30 protein to carbohydrate to fat), it would appear to favourably control/reduce blood glucose postprandially -at least in type 2 diabetics), but this doesn't really tell us much about the mechanisms and chemical/metabolic pathways for each of the different macronutrients independently (ie: protein, fat, carbs).

I also think the 'rabbit starvation' effects of high protein low carb and low fat diets are very interesting counter-arguments against the idea that nearly 60% of 'excess' protein eaten is converted to glucose in the body (ie: the claim that 0.58 per gram of protein can be converted to glucose). This seems an extraordinary amount to me and I would love to know what studies or whatever this is based on!

Kristelle's point about organ meats is a good one, especially since something like liver has far more glycogen carbs than muscle meat!

Did Lex measure carb content in his meals? I need to reread the thread and check...darn, I'm feeling lazy  Roll Eyes  LOL

Still, even with some organ meats his would be a far more carb-controlled environment than all the science articles and studies like those mentioned above, that seem to be all we currently have to go on. Or the ones I've seen at any rate. If anyone has others, pleasums could you post the links?!
lex_rooker

I just had dinner with a friend that I’ve not seen in several years.  He works in the area of doing behavioral studies on government and private grants.  We discussed my experiment and he pointed out that my proposal changes 2 elements at the same time – fat/protein ratio & calories.  He felt that a better approach was to change the fat/protein ratio but reduce the volume of food eaten to keep calories the same.  This way only one variable is changing.

I currently eat between 800 and 850 grams of food per day, and at the 18% fat level this works out to 1900 to 2000 calories.  When I raise the fat to 30% I would have to reduce the volume of food to between 600 and 625 grams per day to keep the calories the same.  This is a reduction of almost half a pound of food which seems like a lot.

This change would also drop the amount of protein from 140gm/day to 90gm/day which is right at the calculated minimum for my weight based on 1.2 grams per kilogram of body weight.

In the light of “Famous Jeff’s” MAM experiment where he ate everything including the kitchen sink and didn’t gain an ounce, I’m wondering if maybe holding calories constant while changing the fat/protein ratio might not be a better approach for my experiment.

What do you think?

Lex
lex_rooker

Billi-Jean - I really don't have a way to measure the carb content of the food I eat.  The pet food mix varies from order to order based on what's available at the time so I'm sure that if varies some.

There are carbs in some meat tissues but not sure if it is significant enough to worry over.  If there were 10 grams or so then the total calories from carbs would be about 40 which works out to 2% of total calories eaten per day.

If anyone has a way of measuring this accurately I'm willing to give it a try.  Calculations are out because there is no way to know how much of any given type of organ meat is in each mix.
jeff

Quote:
In the light of “Famous Jeff’s” MAM experiment where he ate everything including the kitchen sink and didn’t gain an ounce, I’m wondering if maybe holding calories constant while changing the fat/protein ratio might not be a better approach for my experiment.

What do you think?

Yes, I think holding calories constant while changing the fat/protein ratio is the way to go.
lex_rooker

Finally got a useable picture and it only took me an hour to figure out how to add it to my profile!
Tracy

Nice pic! You look good ;)

You know, I buy a meat/organ mix for my cats and have often thought about eating some. I'm not going to, but I've thought about it. And here you are, eating my cat food!

I agree that holding calories constant would be the thing to do as well, at least for a bit. The increase in fat may make up for the decrease in total food volume.
Craig

I'm glad you're doing this experiment, Lex!  My glucometer is off from consecutive test to test as well. It usually starts out higher, then goes lower until I get readings within ten points or so of each other. It also registers  higher than what my lab test said it was. It was never in the 80's but always around 100 or higher when I measured it. It's now putting me in the 110's to  120's.

At least you'll have a baseline from which to measure.

Craig
lex_rooker

Craig,
I've found that how you take the measurement is critical to getting consistent results.  In talking with the manufacturer's rep for the meter I'm using, he stated that the actual amount of glucose is so small in the sample that just about anything will mess up the reading.

1st you must wash you hands letting water run over the finger(s) you are going to test to assure you remove all traces of soap, sweat or any other possible contamination.  I do a final rinse in distilled water and then let drip dry so that I don’t get any contamination from a hand towel.  I suppose using a fresh paper towel would be OK.

Then clean the area with pure alcohol or a prepackaged wipe meant for the purpose and let it dry before you use the lancet.  Don't use the fancy stuff with the mint or other additives.  Any contamination on your finger tip, including dried sweat, will throw the reading off.

Lower your hand and swing it back and forth half a dozen times to let centrifugal force send blood to your finger tips.  If you do this correctly your finger tips will get a little darker in color from the extra blood.

Now put the test strip in your meter so that it has completed its internal calibration routine by the time your blood droplet is ready.  You don't want to let any moisture evaporate from the blood sample while you wait for the test strip to cycle.  This will really throw the reading off by 10 points or more.

While the fresh test strip is cycling, prick your finger tip with the lancet.  The arm swinging should have created a large enough sample but if it needs a bit of help apply pressure BEHIND the first knuckle.  Never squeeze the pad of the finger tip as this will force Interstitial Fluid into the sample and the reading will be off by as much as 20 points or more.

Take the reading immediately - within 5 seconds from the time you punctured the finger tip.  Any longer and the error will mount quickly.

If you need to take a second reading you must start over with the alcohol, swinging the hand, and then make a new puncture as any blood from the old one will now either have started to evaporate or be full of Interstitial Fluid and the reading will be way off.

Following this procedure gives me readings that are often within 2-3 points of each other.  

Like you, I have no idea of the absolute accuracy of my meter, or of differences between batches of test strips - even if they have the same calibration number.  What you should find is that within the same bottle of test strips the readings will be pretty consistent - within 5 points when consecutive readings are taken.  You will also find the occasional test strip within a batch that gives a bad reading but I usually only find one or two out of a bottle of 50.

Hope this helps,

Lex
Craig

Thanks Lex,

Yes, that does help and I'll be sure to do that from now on. I have always washed my hands but not shaken my fingers or used different prick points and I always pricked my finger first, before I inserted the test strip.

Craig
lex_rooker

Glad you found it useful.  I've been forced to learn more than I ever wanted to know about this stuff when I took on this experiment.

Don't just shake your hand.  You swing your whole arm low and back and forth like a pendulum but exaggerate it to create a good bit of centrifugal force.

Lex
Nicola

Lex, you show a grate charisma Idea !

Nicola
Billi-Jean

Welcome to the bus Craig!!  Sunny Great to have you here!  Sunny
Billi-Jean

lex_rooker wrote:
Finally got a useable picture and it only took me an hour to figure out how to add it to my profile!


LOL I can relate to that!
Lauren

Lex,

What will you add to your diet to up the fat content? Will you just eat fattier meats or will you add other fats?

I look forward to following your experiment.

Lauren
lex_rooker

Lauren - I'll be adding beef suet.  I need everything to be consistent and measurable.

I had a conversation with my sister this weekend.  She has converted her German Sheppard’s to a raw meat diet.  Over the months she has observed that when the dogs are receiving the correct amount of food, their stools are small, well formed, and hard.

If she increases the amount of food so that it is more than they need, then the stools become soft and pasty and leave "skid marks" on the shovel when she picks them up.  The volume also seems to increase.

If the food is further increased, say they make an error and feed the dogs double the food they normally require, then the stools become very loose and often runny - like diarrhea - and the volume of the stools increases significantly for a 24 - 36 hour period.

When the food volume is brought down to the correct level the stools immediately revert back to their small, well formed, hard state.

This has me intrigued and I'm going to look into how feasible it will be to measure stool volume and consistency. If I can come up with something that is doable, I may add this to what I'm currently measuring.  After all, we're all wondering where the excess calories go and I'm not sure anyone has actually done an accurate Input vs. Output measurement.

They use a "hat" to collect fecal samples in the hospital.  If I can get a supply of these it may be a simple matter of catching, weighing, and then discarding the whole thing - a rather clean process.

This project becomes more interesting as it progresses.  Any thoughts?

Lex
Billi-Jean

lex_rooker wrote:
This project becomes more interesting as it progresses.  Any thoughts?

Lex


Lex, your wife is a tolerant, understanding soul, I trust? LOL

I think it’s a great idea but I don't know that the subsequent change in the volume that you eat will be enough to show a marked difference in stool volume or composition. Not sure, but it's worth trying, definitely!
You may perhaps have to additionally consider, in that case, the strict monitoring of water intake as well, I would imagine.
jeff

Measuring the volume of the fecal matter is fine, (did I really just say that)  LOL  but will that really tell us anything about the amount of wasted energy?  I'm sure there'd be a way to measure it, but I'd doubt any of us would have that capability in our homes.
Sol

Lex,

I believe that the number of good bugs in our guts has a role in the "look and feel" of our poops. I'm guessing that those of us who eat lots of raw meat, or take probiotics, or eat fermented foods, feed the good bugs which have an influence on the shape and consistency of our poops. But, I don't know what to do with this thought.

Sol
Billi-Jean

jeff wrote:
Measuring the volume of the fecal matter is fine, (did I really just say that)  LOL  but will that really tell us anything about the amount of wasted energy?  I'm sure there'd be a way to measure it, but I'd doubt any of us would have that capability in our homes.


yeah, good point, jeff.

Feces is basically water, inorganic salts, sloughed off epithelial cells from the mucosa lining of the GI tract, bacteria, products of bacterial decomposition, indigestible parts of food and unabsorbed digested materials.

So maybe all we'd see is whether some changes occur with regards to total amount of protein and fat that doesn't get absorbed. But I imagine with someone like Lex, who doesn't have fibre etc to disrupt absorption or to speed up passage of food through the gut, most of what he eats will get absorbed.

So I change my mind from my initial comment to say..."Yeah, what jeff said!"  Grin
Sol

Lex,

You can measure volume of poop by noting how much water your poop displaces in a big glass measuring cup. You'll have to somehow hold it under water if it floats. Disposable chopsticks work great for playing with your poop. If it all falls apart and floats then it'd be hard to even measure its volume.

You might want to develop a subjective measure of consistency: 1 - hard rubber, 2 - soft rubber, 3 - barely holds together, 4 - doesn't hold together.

You might also want to note color and buoyancy, also subjectively.

Sol
jeff

Sol wrote:
Lex,

You can measure volume of poop by noting how much water your poop displaces in a big glass measuring cup. You'll have to somehow hold it under water if it floats. Disposable chopsticks work great for playing with your poop. If it all falls apart and floats then it'd be hard to even measure its volume.

You might want to develop a subjective measure of consistency: 1 - hard rubber, 2 - soft rubber, 3 - barely holds together, 4 - doesn't hold together.

You might also want to note color and buoyancy, also subjectively.

Sol

Ok, this is just getting too weird.  LOL   I'm headed to the back of the bus with some Kool-Aid, warm melted butter, and maybe a Heather or two...... Outta Here
Billi-Jean

Sol wrote:
Disposable chopsticks work great for playing with your poop. If it all falls apart and floats then it'd be hard to even measure its volume.


LOL oh dear...I feel the giggles coming on...  ROTFL  sorry, okay, yes, I know this is serious. I shall behave forthwith...
jeff

Billi-Jean wrote:
Sol wrote:
Disposable chopsticks work great for playing with your poop. If it all falls apart and floats then it'd be hard to even measure its volume.


LOL oh dear...I feel the giggles coming on...  ROTFL  sorry, okay, yes, I know this is serious. I shall behave forthwith...

Perhaps you'd like to join me and the Heathers?  Devil
Billi-Jean

I'll race you!  Outta Here
jeff

Billi-Jean wrote:
I'll race you!  Outta Here

Ready.....set......go!!!!   Outta Here
lex_rooker

Sol wrote:

You can measure volume of poop by noting how much water your poop displaces in a big glass measuring cup.
Sol


Not sure this would be any different than weight.  Any water contained in the matter would be added to the water in the measuring device and increase the level.  For this to work you'd have to dry the material and then add it back to water.  Again, if it were dry then simple weight would tell you the same thing.  Or am I missing something.

Sol wrote:

You might want to develop a subjective measure of consistency: 1 - hard rubber, 2 - soft rubber, 3 - barely holds together, 4 - doesn't hold together.

You might also want to note color and buoyancy, also subjectively.
Sol


This seems like a reasonable approach, although I would expect color and bouyancy to stay realatively constant as the composition of the food will always be the same except at the time of the change, and then it should remain consistent after that until another change is made.

I would also propose a 2 step experiment here.  First change would be to change fat/protein ratio while keeping calories about the same.  Run this for as many weeks necessary until all measured parameters are stable and nothing is changing.  Then increase the volume of food from 600 grams/day to 1kg/day which is a 65% increase in volume and calories if I stay with the high fat mixture.  One would think that this should cause some type of change.
Craig

lex_rooker wrote:
Lauren - I'll be adding beef suet.  I need everything to be consistent and measurable.

I had a conversation with my sister this weekend.  She has converted her German Sheppard’s to a raw meat diet.  Over the months she has observed that when the dogs are receiving the correct amount of food, their stools are small, well formed, and hard.

If she increases the amount of food so that it is more than they need, then the stools become soft and pasty and leave "skid marks" on the shovel when she picks them up.  The volume also seems to increase.

If the food is further increased, say they make an error and feed the dogs double the food they normally require, then the stools become very loose and often runny - like diarrhea - and the volume of the stools increases significantly for a 24 - 36 hour period.

When the food volume is brought down to the correct level the stools immediately revert back to their small, well formed, hard state.

This has me intrigued and I'm going to look into how feasible it will be to measure stool volume and consistency. If I can come up with something that is doable, I may add this to what I'm currently measuring.  After all, we're all wondering where the excess calories go and I'm not sure anyone has actually done an accurate Input vs. Output measurement.

They use a "hat" to collect fecal samples in the hospital.  If I can get a supply of these it may be a simple matter of catching, weighing, and then discarding the whole thing - a rather clean process.

This project becomes more interesting as it progresses.  Any thoughts?

Lex


Interesting observation. I suspect that if her dogs went without eating for a few days, those large meals would be almost fully absorbed and their stools would be normal.

I like your methods. In the mean time, have you thought about fasting for a day to see if your drop in BG would come back up after the missed meal? This would tell us if it's your body clock doing this. if it stays low, I think we have our answer.

I know you once changed your feeding time but that would throw a wrench into your circadian rhythm, which sounds like what happened.

When I did IF (eating once every other day) my BG remained relatively consistent.

Craig
lex_rooker

Craig wrote:
In the mean time, have you thought about fasting for a day to see if your drop in BG would come back up after the missed meal? This would tell us if it's your body clock doing this. if it stays low, I think we have our answer. Craig

Never thought about doing a one day fast as I'm not really into scheduled Intermittent Fasting.  I did the fasting thing 25 years ago and after a 31 dayer where I dropped to 95 lbs, and it took about 2 years for me to recover, I never got enthusiastic about it again.

I suppose I could do 1 day - in the interest of sciense of course....

Lex
Craig

One day won't hurt you. I suspect you'll be hungry around your usual time but then it'd subside later on. My BG didn't fall while I was fasting but I wasn't on a schedule either. It fact, the time before last, when I donated blood, It was on a fasting day. I had no light-headedness, didn't pass out. I was just fine as usual. Then I went to work and was still fine. Just had some water.

I don't do it anymore because I couldn't eat enough on my eating days to make up for my fasting days. As it is, I can't eat as much as you lean people! :-)

Actually, eating once a day is a form of IF. This thread is so long, I'm not sure if anyone covered that. I know you had a question about it.

Craig
Nicola

This is getting very interesting; eating cooked meat and fat would give a different picture right to the stool!

Many don't talk about urin and stool; I am not so shore but I think many just put up with things and find their own answer. If dogs can put up with different feces (from cooked vs. raw), then humans will too!

Lex, let us know if you LIKE eating more fat when you are doing this! Food is a part of our emotional life.

Nicola

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